Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA

Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.

If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse sperm...

Inactivated African Horse Sickness Virus Cell Culture Vaccine

Spermatozoa quality of the tranSgenic rabbit offSpring

The objective of this study was to evaluate the spermatozoa quality of the F4 F5 generation of transgenic rabbit males carrying the mWAP-hFVIII gene in comparison to non-transgenic individuals and previous transgenic generations. Semen samples from 4 transgenic rabbits and 10 randomly selected non-transgenic males of the same breed and age were collected once a week (5 ejaculates per male were ...

Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation.

A sperm-specific phospholipase C-zeta (PLCζ) is believed to play an essential role in oocyte activation during mammalian fertilization. Sperm PLCζ has been shown to trigger a prolonged series of repetitive Ca(2+) transients or oscillations in oocytes that precede activation. This remarkable intracellular Ca(2+) signalling phenomenon is a distinctive characteristic observed during in vitro ferti...

Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring.

Genomic imprinting occurs in both male and female gametes during gametogenesis, but the exact time when imprinting begins and ends is unknown. In the present study we injected nuclei of testicular spermatozoa and round spermatids into mature mouse oocytes to see whether these nuclei are able to participate in syngamy and normal embryonic development. If the injected oocytes develop into normal ...